By G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)
The use of the time period "advanced" within the name of this booklet is a bit of ar bitrary and extremely a lot relative with appreciate to time. Many innovations that have been thought of on the "cutting part" of ultrastructural method quite a few years in the past at the moment are rou tin ely utilized in a variety of laboratories. you can actually cite freeze-fracture, cryothin sectioning, or certainly lots of the box of experiment ning electron microscopy as concrete examples. hence using the time period "ad vanced concepts" needs to be interpreted with reference to the current state-of-the-art, and comes in handy basically in informing the capability reader that this quantity isn't really a primer for use as an preliminary advent into simple organic elec tron microscopy. many fantastic volumes have stuffed that area of interest long ago few years, and it isn't meant that this modest booklet be a whole com pendium of the sphere. moreover, any constrained choice of papers on advanc ed options inevitably displays the personal tastes and arbitrary whims of the editor, thereby apart from many both very important techniques which the an expert reader will with no trouble determine. the 1st quantity of this sequence seemed nearly 5 years in the past and illustrated innovations that have been inspiration to symbolize complicated and but ba sically morphological tools for gaining elevated ultrastructural informa tion from organic specimens. the current quantity, nevertheless, stresses ideas which supply particular physicochemical facts at the speci mens as well as the structural information.
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Extra resources for Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes
The procedures discussed will include preparation of cell suspensions prior to reaction with labeled antibody, reaction of cells with labeled antibody, and specialized preparation of cells after reaction with labeled antibodies. D. Iodination of Antibody Recently, radioactive iodine C25 I) has been used as an ultrastructural label for antibody. It is especially useful for morphologic studies in that it can be used for both light-microscopic and ultrastructural evaluation. This has the advantage of allowing one to observe results almost immediately with the light microscope including the quantitation of large numbers of cells.
NICOLSON Nicolson, G. : Ferritin-conjugated plant agglutinins as specific saccharide stains for electron microscopy: Application to saccharides bound to cell membranes. Proc. Natl. Acad. Sci. S. 68,942 - 945 (1971) Nicolson, G. , Singer, S. ]. Cell BioI. 60, 236 - 248 (1974) Nicolson, G. , Smith,]. ]. Cell BioI. (submitted, 1977) Nicolson, G. : Mobility and the restriction of mobility of plasma membrane lectin-binding components. Science 184, 1294 -1296 (1974) Nicolson, G. ]. Cell BioI. : Lymphocyte transformation induced by concanavalin A and its reversion by methyl-alpha-D-mannopyranoside.
Turner, B. L. ). New York: Academic Press 1971, pp. : Studies on the ultrastructure of concanavalin A receptor sites at the surface of normal cells and cells transformed by oncogenic viruses. Inc. J. : Some properties of purified phytohemagglutinin from Lens culinaris seeds. Biochim. Biophys. Acta 221,514 - 521 (1970) Trowbridge, 1. : Isolation and chemical characterization of a mitogenic lectin from Pisum sativum. J. BioI. Chern. , Pardoe, G. : On the specificity of lectins with a broad agglutination spectrum.